Multi‐antibiotics resistance phenotype of pathogenic <i>Vibrio parahaemolyticus</i> isolated from acute hepatopancreatic necrosis disease in <i>Litopenaeus vannamei</i> farmed in the Mekong Delta

Vibrio parahaemolyticus is a significant causal agent of acute hepatopancreatic necrosis disease (AHPND), with huge production losses white leg shrimp, Litopenaeus vannamei, cultivated globally, including aquaculture farms in the Mekong Delta Vietnam. Controlling this critical because worldwide expansion antimicrobial-resistant V. isolates. The purpose study was to determine frequency multi-antibiotic resistance (MAR) isolated from AHPND shrimp extensively Bac Lieu Province (Mekong Delta). Based on biochemical tests and toxR-PCR positive detections, 34 isolates were identified. Antibiotic susceptibility examination revealed that most phenotypically multidrug resistant, ceftazidime (100%) amoxicillin (97.06%), followed by colistin (74%) erythromycin (65%). These highly sensitive doxycycline (94%), florfenicol flumequine (71%). There 27 distinct MAR phenotypes detected among isolates, 14.71% exhibiting antibiotic profile AMO-CEF-CEP-COL-ERY, 11.76% for each profiles AMO-CEP-TET-COL AMO–CEP–COL. In particular, 25 (75%) resistant at least 5 (of 12) antibiotics tested. A hierarchical clustering analysis antibiotic-resistant indicated cross-transmission across its ability survive aquatic environments extended periods (over 3 months). Our findings support hypothesis variety MARPs result human activities. Environmentally friendly therapy strategies should be used prophylaxis treatment infection.